公山羊Protamine 1 mRNA表达特性及与精液品质相关性的研究

doi:10.11843/j.issn.0366-6964.2016.07.007

Abstract

Abstract:

The aims of this study were to evaluate the expression profiles of Protamine 1（PRM1） mRNA in reproductive organs of bucks and to analyze the correlation between expression of PRM1 mRNA in spermatozoa and semen quality index.In this experiment，mRNA expression was detected by real-time fluorescent quantitative PCR.Cellular localization of PRM1 in testis was examined by immunohistochemistry.The correlation was analyzed by GraphPad Prism 5 software.The results showed that：the expression of PRM1 mRNA in testis was the highest in all tissue detected，which was 247 times higher than that in the epididymal caput（P＜0.05）.The expression of PRM1 mRNA in epididymal caput was significantly higher than that in epididymal corpus and cauda（P<0.05）.The expression of PRM1 mRNA in other tissues was very few.The expression of PRM1 mRNA in testis was gradually increased with the increase of ages in one year old.The PRM1 mRNA expression in testis at 12 months old were significantly higher than that at 9 months old（P＜0.05）.The PRM1 mRNA expression in testis at 9 months old was significantly higher than that in other ages.Concentration of PRM1 mRNA in sperm was positively correlated with the sperm motility （R2=0.586 0，P=0.000 5） and sperm concentration （R2=0.442 2，P=0.004 9）.PRM1 was located in elongating spermatids and spermatozoa in testis of bucks.There was no positive signal in other cells of testis.This study indicated that the expression of PRM1 mRNA was spatial and temporal-specific pattern.The sperm PRM1 mRNA content could be index of fertility of bucks.

CLC Number:

S827.2

ZHANG Chun-xiang，ZHAO Hui，GUO Li-na，ZHENG Ya-lin，ZHANG Cai-xia，LIU Wen-zhong，QIAO Li-ying，REN You-she. Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1354-1362.

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Expression Profiles of Protamine 1 mRNA and Correlation with Index of Semen Quality in Bucks

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